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dhc1p d18  (Croda International Plc)

 
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    Structured Review

    Croda International Plc dhc1p d18
    Regulation of ORMDLs stability in human RPE‐1 cells and mouse BMMCs. (A) Levels of ORMDLs, SPTLC1, SPTLC2, and total <t>d18:1</t> ceramides (measured by LC‐ESI‐MS/MS) were assessed in human RPE‐1 cells and mouse BMMCs after 24‐h treatment with 10 μM myriocin or 10 μM FB 1 , and compared with untreated controls. (B) Quantification of ORMDLs, SPTLC1, SPTLC2, and the LC3‐II/LC3‐I ratio was performed in untreated RPE‐1 cells and BMMCs, and compared with cells treated for 24 h with 50 μM CQ or 10 μM MG132, as determined by immunoblotting. (C) Effects of p97/VCP inhibition by CB‐5083 on ORMDLs, SPTLC1, SPTLC2, the LC3‐II/LC3‐I ratio, ATF4, and p62 levels were assessed in RPE‐1 cells and BMMCs. Protein levels in (A‐C) were normalized to total protein loading using Ponceau S staining. Data are presented as geometric mean ± GSEM ( N = 4). Statistical analysis was performed using one‐way ANOVA with Tukey's post hoc test. Significance levels: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****); n.s., not significant.
    Dhc1p D18, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dhc1p+d18/C16+Dihydroceramide-1-Phosphate/pmc12974560-239-25-38
    Average 94 stars, based on 1 article reviews
    dhc1p d18 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell‐Type Dependent and Tied to Ceramide Homeostasis"

    Article Title: ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell‐Type Dependent and Tied to Ceramide Homeostasis

    Journal: The FASEB Journal

    doi: 10.1096/fj.202502924RR

    Regulation of ORMDLs stability in human RPE‐1 cells and mouse BMMCs. (A) Levels of ORMDLs, SPTLC1, SPTLC2, and total d18:1 ceramides (measured by LC‐ESI‐MS/MS) were assessed in human RPE‐1 cells and mouse BMMCs after 24‐h treatment with 10 μM myriocin or 10 μM FB 1 , and compared with untreated controls. (B) Quantification of ORMDLs, SPTLC1, SPTLC2, and the LC3‐II/LC3‐I ratio was performed in untreated RPE‐1 cells and BMMCs, and compared with cells treated for 24 h with 50 μM CQ or 10 μM MG132, as determined by immunoblotting. (C) Effects of p97/VCP inhibition by CB‐5083 on ORMDLs, SPTLC1, SPTLC2, the LC3‐II/LC3‐I ratio, ATF4, and p62 levels were assessed in RPE‐1 cells and BMMCs. Protein levels in (A‐C) were normalized to total protein loading using Ponceau S staining. Data are presented as geometric mean ± GSEM ( N = 4). Statistical analysis was performed using one‐way ANOVA with Tukey's post hoc test. Significance levels: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****); n.s., not significant.
    Figure Legend Snippet: Regulation of ORMDLs stability in human RPE‐1 cells and mouse BMMCs. (A) Levels of ORMDLs, SPTLC1, SPTLC2, and total d18:1 ceramides (measured by LC‐ESI‐MS/MS) were assessed in human RPE‐1 cells and mouse BMMCs after 24‐h treatment with 10 μM myriocin or 10 μM FB 1 , and compared with untreated controls. (B) Quantification of ORMDLs, SPTLC1, SPTLC2, and the LC3‐II/LC3‐I ratio was performed in untreated RPE‐1 cells and BMMCs, and compared with cells treated for 24 h with 50 μM CQ or 10 μM MG132, as determined by immunoblotting. (C) Effects of p97/VCP inhibition by CB‐5083 on ORMDLs, SPTLC1, SPTLC2, the LC3‐II/LC3‐I ratio, ATF4, and p62 levels were assessed in RPE‐1 cells and BMMCs. Protein levels in (A‐C) were normalized to total protein loading using Ponceau S staining. Data are presented as geometric mean ± GSEM ( N = 4). Statistical analysis was performed using one‐way ANOVA with Tukey's post hoc test. Significance levels: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****); n.s., not significant.

    Techniques Used: Tandem Mass Spectroscopy, Western Blot, Inhibition, Staining

    Related Articles

    Tandem Mass Spectroscopy:

    Article Title: ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell‐Type Dependent and Tied to Ceramide Homeostasis
    Article Snippet: When switching between analyses with different ion‐optics settings within a single run, a settling time of 700 ms was applied.When switching between analyses with different ion‐optics settings within a single run, a settling time of 700 ms was applied.. Sphingolipid concentrations of S1P, dhC1P, and C1P were determined by single‐point calibration using external standards [ ]: 50 ng S1P d18:1 (Cat# 860492), 50 ng dhC1P d18:0/C16:0 (Cat# 860522), and 50 ng C1P d18:1/C16:0 (Cat# 860533), all from Avanti Polar Lipids.. Values were corrected by the signal ratio to the corresponding internal standards added during the extraction procedure.Values were corrected by the signal ratio to the corresponding internal standards added during the extraction procedure.

    Western Blot:

    Article Title: ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell‐Type Dependent and Tied to Ceramide Homeostasis
    Article Snippet: When switching between analyses with different ion‐optics settings within a single run, a settling time of 700 ms was applied.When switching between analyses with different ion‐optics settings within a single run, a settling time of 700 ms was applied.. Sphingolipid concentrations of S1P, dhC1P, and C1P were determined by single‐point calibration using external standards [ ]: 50 ng S1P d18:1 (Cat# 860492), 50 ng dhC1P d18:0/C16:0 (Cat# 860522), and 50 ng C1P d18:1/C16:0 (Cat# 860533), all from Avanti Polar Lipids.. Values were corrected by the signal ratio to the corresponding internal standards added during the extraction procedure.Values were corrected by the signal ratio to the corresponding internal standards added during the extraction procedure.

    Inhibition:

    Article Title: ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell‐Type Dependent and Tied to Ceramide Homeostasis
    Article Snippet: When switching between analyses with different ion‐optics settings within a single run, a settling time of 700 ms was applied.When switching between analyses with different ion‐optics settings within a single run, a settling time of 700 ms was applied.. Sphingolipid concentrations of S1P, dhC1P, and C1P were determined by single‐point calibration using external standards [ ]: 50 ng S1P d18:1 (Cat# 860492), 50 ng dhC1P d18:0/C16:0 (Cat# 860522), and 50 ng C1P d18:1/C16:0 (Cat# 860533), all from Avanti Polar Lipids.. Values were corrected by the signal ratio to the corresponding internal standards added during the extraction procedure.Values were corrected by the signal ratio to the corresponding internal standards added during the extraction procedure.

    Staining:

    Article Title: ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell‐Type Dependent and Tied to Ceramide Homeostasis
    Article Snippet: When switching between analyses with different ion‐optics settings within a single run, a settling time of 700 ms was applied.When switching between analyses with different ion‐optics settings within a single run, a settling time of 700 ms was applied.. Sphingolipid concentrations of S1P, dhC1P, and C1P were determined by single‐point calibration using external standards [ ]: 50 ng S1P d18:1 (Cat# 860492), 50 ng dhC1P d18:0/C16:0 (Cat# 860522), and 50 ng C1P d18:1/C16:0 (Cat# 860533), all from Avanti Polar Lipids.. Values were corrected by the signal ratio to the corresponding internal standards added during the extraction procedure.Values were corrected by the signal ratio to the corresponding internal standards added during the extraction procedure.



    Similar Products

    dhc1p d18  (Croda International Plc)
    94
    Croda International Plc dhc1p d18
    Regulation of ORMDLs stability in human RPE‐1 cells and mouse BMMCs. (A) Levels of ORMDLs, SPTLC1, SPTLC2, and total <t>d18:1</t> ceramides (measured by LC‐ESI‐MS/MS) were assessed in human RPE‐1 cells and mouse BMMCs after 24‐h treatment with 10 μM myriocin or 10 μM FB 1 , and compared with untreated controls. (B) Quantification of ORMDLs, SPTLC1, SPTLC2, and the LC3‐II/LC3‐I ratio was performed in untreated RPE‐1 cells and BMMCs, and compared with cells treated for 24 h with 50 μM CQ or 10 μM MG132, as determined by immunoblotting. (C) Effects of p97/VCP inhibition by CB‐5083 on ORMDLs, SPTLC1, SPTLC2, the LC3‐II/LC3‐I ratio, ATF4, and p62 levels were assessed in RPE‐1 cells and BMMCs. Protein levels in (A‐C) were normalized to total protein loading using Ponceau S staining. Data are presented as geometric mean ± GSEM ( N = 4). Statistical analysis was performed using one‐way ANOVA with Tukey's post hoc test. Significance levels: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****); n.s., not significant.
    Dhc1p D18, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dhc1p+d18/C16+Dihydroceramide-1-Phosphate/pmc12974560-239-25-38
    Average 94 stars, based on 1 article reviews
    dhc1p d18 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Regulation of ORMDLs stability in human RPE‐1 cells and mouse BMMCs. (A) Levels of ORMDLs, SPTLC1, SPTLC2, and total d18:1 ceramides (measured by LC‐ESI‐MS/MS) were assessed in human RPE‐1 cells and mouse BMMCs after 24‐h treatment with 10 μM myriocin or 10 μM FB 1 , and compared with untreated controls. (B) Quantification of ORMDLs, SPTLC1, SPTLC2, and the LC3‐II/LC3‐I ratio was performed in untreated RPE‐1 cells and BMMCs, and compared with cells treated for 24 h with 50 μM CQ or 10 μM MG132, as determined by immunoblotting. (C) Effects of p97/VCP inhibition by CB‐5083 on ORMDLs, SPTLC1, SPTLC2, the LC3‐II/LC3‐I ratio, ATF4, and p62 levels were assessed in RPE‐1 cells and BMMCs. Protein levels in (A‐C) were normalized to total protein loading using Ponceau S staining. Data are presented as geometric mean ± GSEM ( N = 4). Statistical analysis was performed using one‐way ANOVA with Tukey's post hoc test. Significance levels: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****); n.s., not significant.

    Journal: The FASEB Journal

    Article Title: ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell‐Type Dependent and Tied to Ceramide Homeostasis

    doi: 10.1096/fj.202502924RR

    Figure Lengend Snippet: Regulation of ORMDLs stability in human RPE‐1 cells and mouse BMMCs. (A) Levels of ORMDLs, SPTLC1, SPTLC2, and total d18:1 ceramides (measured by LC‐ESI‐MS/MS) were assessed in human RPE‐1 cells and mouse BMMCs after 24‐h treatment with 10 μM myriocin or 10 μM FB 1 , and compared with untreated controls. (B) Quantification of ORMDLs, SPTLC1, SPTLC2, and the LC3‐II/LC3‐I ratio was performed in untreated RPE‐1 cells and BMMCs, and compared with cells treated for 24 h with 50 μM CQ or 10 μM MG132, as determined by immunoblotting. (C) Effects of p97/VCP inhibition by CB‐5083 on ORMDLs, SPTLC1, SPTLC2, the LC3‐II/LC3‐I ratio, ATF4, and p62 levels were assessed in RPE‐1 cells and BMMCs. Protein levels in (A‐C) were normalized to total protein loading using Ponceau S staining. Data are presented as geometric mean ± GSEM ( N = 4). Statistical analysis was performed using one‐way ANOVA with Tukey's post hoc test. Significance levels: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****); n.s., not significant.

    Article Snippet: Sphingolipid concentrations of S1P, dhC1P, and C1P were determined by single‐point calibration using external standards [ ]: 50 ng S1P d18:1 (Cat# 860492), 50 ng dhC1P d18:0/C16:0 (Cat# 860522), and 50 ng C1P d18:1/C16:0 (Cat# 860533), all from Avanti Polar Lipids.

    Techniques: Tandem Mass Spectroscopy, Western Blot, Inhibition, Staining